Anti-Cathepsin B Anticorps (A121144)

Name: Anti-Cathepsin B Antibody
Brand: Antibodies.com
SKU: A121144
Price: 480.0 USD
Availability: InStock

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$480

Anticorps polyclonal de chèvre contre Cathepsin B pour ELISA, IF, IHC et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Cathepsin B Antibody

Description du produit

Goat polyclonal antibody to Cathepsin B.

Applications

ELISA, IF, IHC, Flow Cytometry

Recommander des dilutions

ELISA: 1:64,000, IHC: 5µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human Cathepsin B.

Séquence

C-DELVNYVNKRNT

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Amyloid precursor protein secretase, APP secretase, APPS, CATB_HUMAN, Cathepsin B heavy chain, Cathepsin B1, CathepsinB, CPSB, CTSB, cysteine protease, OTTHUMP00000116009, OTTHUMP00000229510, OTTHUMP00000229511, OTTHUMP00000229512, OTTHUMP00000229514, OTTHUMP00000229515, OTTHUMP00000229516, Preprocathepsin B

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunofluorescence - Anti-Cathepsin B Antibody (A121144)

Cathepsin B expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Cathepsin B Antibody (A121144) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-Cathepsin B Antibody (A121144)

Cathepsin B expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Cathepsin B Antibody (A121144) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-Cathepsin B Antibody (A121144)

Cathepsin B expression in HepG2 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Cathepsin B Antibody (A121144) at 10µg/ml overnight and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-Cathepsin B Antibody (A121144)

Cathepsin B expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Cathepsin B Antibody (A121144) at 5µg/ml and revealed with alkaline phosphatase (AP).