Western blot analysis of extracts of various cell lines, using Anti-Caspase-8 Antibody (A12547) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of Jurkat cells, using Anti-Caspase-8 Antibody (A12547) at 1:1,000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Caspase-8 Antibody (A12547) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Caspase-8 Antibody (A12547) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Caspase-8 Antibody (A12547) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-Caspase-8 Antibody (A12547) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of HepG2 cells using Anti-Caspase-8 Antibody (A12547) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-Caspase-8 Antibody (A12547) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-Caspase-8 Antibody (A12547) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
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