Anti-Caspase-3 Anticorps [ARC0133] (A308661)

Name: Anti-Caspase-3 Antibody [ARC0133]
Brand: Antibodies.com
SKU: A308661
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC0133] de lapin contre Caspase-3 pour WB et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout

Spécifications

Nom du produit

Anti-Caspase-3 Antibody [ARC0133]

Description du produit

Rabbit monoclonal [ARC0133] antibody to Caspase-3.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 29-175 of human Caspase-3 (P42574).

Séquence

SGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETD

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0133

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

17 kDa / 32 kDa / 35 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

A830040C14Rik, Apopain, CASP-3, CASP3, Casp3a, CASP3_HUMAN, Caspase 3, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase-3 subunit p12, CC3, CPP-32, CPP32, CPP32B, Cysteine protease CPP32, EC 3.4.22.56, LICE, mldy, OTTHUMP00000165052, OTTHUMP00000165053, OTTHUMP00000165054, PARP cleavage protease, Procaspase3, Protein Yama, SCA 1, SCA-1, SREBP cleavage activity 1, Yama

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

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Figure 1: Western Blot - Anti-Caspase-3 Antibody [ARC0133] (A308661)

Western blot analysis of various lysates, using Anti-Caspase-3 Antibody [ARC0133] (A308661) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Western Blot - Anti-Caspase-3 Antibody [ARC0133] (A308661)

Western blot analysis of extracts of Rat liver, using Anti-Caspase-3 Antibody [ARC0133] (A308661) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-Caspase-3 Antibody [ARC0133] (A308661)

Western blot analysis of extracts of Jurkat, using Anti-Caspase-3 Antibody [ARC0133] (A308661) at 1:1,000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 4: Western Blot - Anti-Caspase-3 Antibody [ARC0133] (A308661)

Western blot analysis of extracts of L929, using Anti-Caspase-3 Antibody [ARC0133] (A308661) at 1:1,000 dilution. L929 cells were treated by staurosporine(1 uM) for 3 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.