Immunocytochemistry/Immunofluorescence analysis of mouse dissociated hippocampal neurons, fixed in cold 4% paraformaldehyde / 0.2% glutaraldehyde in 0.1M sodium phosphate buffer, using Anti-CaMKII alpha Antibody [6G9] (A305083), at 1:1,000 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at room temperature. Magnification: 10X.
Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-CaMKII alpha Antibody [6G9] (A305083) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Magnification: 40x.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CaMKII alpha Antibody [6G9] (A305083) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Hair follicles, epidermis.
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-CaMKII alpha Antibody [6G9] (A305083), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Nuclear Staining.