Anti-Calpain 2 Antibody (A14127)

Name: Anti-Calpain 2 Antibody
Brand: Antibodies.com
SKU: A14127
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Calpain 2 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Calpain 2 Antibody

Description

Rabbit polyclonal antibody to Calpain 2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 520-700 of human Calpain 2 (NP_001739.2).

Sequence

LEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Calcium-activated neutral proteinase 2, Calpain large polypeptide L2, Calpain M-type, Calpain-2 catalytic subunit, Calpain-2 large subunit, CANP 2, CANPL2, CAPN2, M-calpain, Millimolar-calpain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Calpain 2 Antibody (A14127)

Western blot analysis of extracts of various cell lines, using Anti-Calpain 2 Antibody (A14127). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Western Blot - Anti-Calpain 2 Antibody (A14127)

Western blot analysis of extracts of various cell lines, using Anti-Calpain 2 Antibody (A14127) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunohistochemistry - Anti-Calpain 2 Antibody (A14127)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Calpain 2 Antibody (A14127) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Calpain 2 Antibody (A14127)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Calpain 2 Antibody (A14127) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.