Rabbit polyclonal antibody to Calnexin (phospho Ser583).
Spécificité
This antibody detects endogenous levels of Calnexin only when phosphorylated at Ser583.
Applications
WB, IHC, IF, ELISA
Recommander des dilutions
WB: 1:500-1:1000, ELISA: 1:1000
Reactivité
Human, Mouse, Rat
Immunogène
Synthetic peptide derived from human Calnexin around the phosphorylation site of Ser583 (amino acids 543-592).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Masse moléculaire
67kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CALX_HUMAN, CANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Figure 1: Western Blot - Anti-Calnexin (phospho Ser583) Antibody (A94794)
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30' using Anti-Calnexin (phospho Ser583) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-Calnexin (phospho Ser583) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-Calnexin (phospho Ser583) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1541] (A250400) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250400_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1541] - BSA and Azide free (A253580) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253580_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1543] (A250401) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250401_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1543] - BSA and Azide free (A253581) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253581_1.jpg?profile=product_alternative)