Anti-c-Fos Anticorps [2H2] (A85387)

Name: Anti-c-Fos Antibody [2H2]
Brand: Antibodies.com
SKU: A85387
Price: 185.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab208942). Découvrez plus pour moins cher.

1 Références

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$185

Anticorps monoclonal [2H2] de souris contre c-Fos pour WB, ICC/IF et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-c-Fos Antibody [2H2]

Description du produit

Mouse monoclonal (2H2) antibody to c-Fos.

Applications

WB, ICC/IF, IHC

Recommander des dilutions

WB: 1:500, ICC/IF: 1:500, IHC: 1:500

Reactivité

Human, Rat, Mouse

Immunogène

Recombinant full-length human c-FOS, expressed in and purified from E. coli.

Hôte

Mouse

Clonalité

Monoclonal

Clone

2H2

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Masse moléculaire

50-65 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Activator protein 1, AP 1, C FOS, Cellular oncogene c fos, Cellular oncogene fos, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FBJ murine osteosarcoma viral v fos oncogene homolog, FBJ Osteosarcoma Virus, FOS, FOS protein, FOS_HUMAN, G0 G1 switch regulatory protein 7, G0/G1 switch regulatory protein 7, G0S7, Oncogene FOS, p55, proto oncogene c Fos, Proto oncogene protein c fos, Proto-oncogene c-Fos, v fos FBJ murine osteosarcoma viral oncogene homolog

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunofluorescence - Anti-c-Fos Antibody [2H2] (A85387)

Section of rat hippocampus stained with Anti-c-Fos Antibody (red) and Anti-FOX3/NeuN Antibody (A85403 | green). DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.

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Figure 2: Immunofluorescence - Anti-c-Fos Antibody [2H2] (A85387)

Left: Anti-c-Fos Antibody staining (green) in HeLa cells, which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or PBS treatment (top panel). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with Anti-Vimentin Antibody (A85421 | red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) labeled with Anti-c-Fos Antibody using a standard HRP-DAB (horseradish peroxidase-3,3’-diaminobenzidine) staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with Anti-c-Fos Antibody (red) and Anti-Fox3/NeuN Antibody (A85403 | green) using immuno-fluorescent confocal-microscopy. Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of Anti-c-Fos Antibody staining. Nuclei are labeled with DAPI (blue).

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Figure 3: Western Blot - Anti-c-Fos Antibody [2H2] (A85387)

Western blot analysis of cell lysates using Anti-c-Fos Antibody [2H2] (A85387), at a dilution of 1:1,000, in green, and Anti-GAPDH Antibody (A85377), at a dilution of 1:20,000, in red, used as a loading control. The lanes contain samples of: [1] Protein standards, in red, [2] HeLa cells in serum free media, [3] HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media, [4] rat cortical neurons, and [5] rat cortical neurons treated with membrane depolarization buffer for 5hrs. Multiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten. The single band at 37 kDa represents GAPDH protein.

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Figure 4: Western Blot - Anti-c-Fos Antibody [2H2] (A85387)

Top panel: Western blot analysis of c-Fos expression in HeLa cells using Anti-c-Fos Antibody. Lane 1: HeLa cells were serum-starved for 36 hours. 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. Anti-c-Fos Antibody recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with Anti-GAPDH Antibody (A85382), used as loading control.

Références pour cet ID de clone (1)

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Regulatory mechanism of melatonin on the retinal ganglion cell photoreaction in mice.

Références : Anti-c-Fos Antibody [2H2] (ab208942)

Résumé :

The study aimed to analyze the regulatory mechanism of melatonin (MLT) on the retinal ganglion cell photoreaction in mice. Forty-eight, 3-week-old healthy ICR mice, regardless of gender, were randomly divided into 4 groups. Group A was exposed to an illumination/dark time of 0 h/24 h, 6 h/18 h in group B, 12 h/12 h in group C and 18 h/6 h in group D, for up to 6 weeks. Four mice in each group were sacrificed at week 1, 3 and 6, respectively, for harvesting of retinal ganglion cells. ELISA was used to detect nocturnal plasma MLT levels at midnight. Immunohistochemistry was used to detect the expression of the retinal MLT receptor and the expression levels of inducible nitric oxide synthase (iNOS) and c-fos protein. The plasma MLT levels, MLT receptor levels and c-fos protein expression levels of group C, after 1, 3 and 6 weeks of light application, were the highest, followed by groups B and D, while group A had the lowest levels. For each illumination time, the iNOS levels of group C were the lowest and group A was the highest. Differences were all statistically significant (P<0.05). In conclusion, appropriate illumination regimens can increase c-fos protein, decrease iNOS activity and regulate the physiological activities of the retinal ganglion cells by regulating the expressions of MLT and its receptor.

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