Anti-Brd4 (phospho Thr204) Anticorps [ARC50960] (A305446)

Name: Anti-Brd4 (phospho Thr204) Antibody [ARC50960]
Brand: Antibodies.com
SKU: A305446
Price: 540.0 USD
Availability: InStock

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$540

Anticorps monoclonal [ARC50960] de lapin contre Brd4 (phospho Thr204) pour WB.

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Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Brd4 (phospho Thr204) Antibody [ARC50960]

Description du produit

Rabbit monoclonal [ARC50960] antibody to Brd4 (phospho Thr204).

Applications

Recommander des dilutions

WB: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1).

Séquence

ASTPP

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC50960

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

200 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

BRD4-NUT FUSION, BRD4-NUT fusion oncoprotein, BRD4_HUMAN, Bromodomain containing 4, bromodomain containing protein 4, Bromodomain-containing protein 4, CAP, chromosome associated protein, HUNK1, HUNKI, MCAP, Mitotic chromosome-associated protein, Protein HUNK1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446)

Western blot analysis of extracts of various cell lines, using Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446) at 1:1,000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) for 30 minutes with NaCl (400mM) after serum-starvation 16-20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.