Nous Contacter
+1 (314) 370-6046 ouNous Contacter
Visit our English Website
Antibodies.com LogoAntibodies.com Logo
Se connecter / S'inscrireMon Compte
0

BLNK (phospho Tyr84) Cell Based Kit Elisa (A102451)

Nom du produit
BLNK (phospho Tyr84) Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This BLNK (phospho Tyr84) Cell Based ELISA Kit allows for the detection of BLNK (phospho Tyr84) and the effects that certain stimulation conditions have on BLNK (phospho Tyr84) expression in different cell lines. Qualitative determination of BLNK (phospho Tyr84) concentration is achieved by an indirect ELISA format. In essence, the BLNK (phospho Tyr84) is captured by Anti-BLNK (phospho Tyr84) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this BLNK (phospho Tyr84) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-BLNK Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
AGM4, B cell adapter containing SH2 domain protein, B cell adapter containing Src homology 2 domain protein, B cell adaptor containing SH2 domain, B cell linker, B cell linker protein, B cell-specific adaptor protein, B-cell activation, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, B-cell linker protein, BASH, Bca, BLNK s, BLNK_HUMAN, Cytoplasmic adapter protein, Ly 57, Ly57, Lymphocyte antigen 57, Lyw 57, Lyw57, MGC111051, SH2 domain-containing leukocyte protein, 65-KD, SLP 65, SLP-65, SLP65, Src homology 2 domain containing leukocyte protein of 65 kDa, Src homology 2 domain-containing leukocyte protein of 65 kDa, Src homology [SH2] domain-containing leukocyte protein of 65 kD
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783)
Western blot analysis of extracts from K562 cells treated with starved 24h, using Anti-BLNK (phopho Tyr84) Antibody.
Protocol Diagram - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783)
A diagram showing the protocol for BLNK (phospho Tyr84) Cell Based ELISA Kit.
Protocol Summary - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783)
A summary of the protocol for BLNK (phospho Tyr84) Cell Based ELISA Kit.
Western Blot - Control Antibody - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783)
The mouse monoclonal antibody to GAPDH is used as a positive control inBLNK (phospho Tyr84) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Menu Principal Nous Contacter 0Caisse
Haut