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BIM Cell Based Kit Elisa (A103312)

Nom du produit
BIM Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This BIM Cell Based ELISA Kit allows for the detection of BIM and the effects that certain stimulation conditions have on BIM expression in different cell lines. Qualitative determination of BIM concentration is achieved by an indirect ELISA format. In essence, the BIM is captured by Anti-BIM Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this BIM Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
B2L11_HUMAN, BAM, Bcl 2 interacting protein Bim, Bcl 2 related ovarian death agonist, Bcl-2-like protein 11, BCL2 interacting mediator of cell death, BCL2 like 11, BCL2 like 11 (apoptosis facilitator), BCL2 like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11, Bcl2l11, BIM alpha6, BIM beta6, BIM beta7, BimEL, BimL, BOD
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - BIM Cell Based ELISA Kit (CB5074) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - BIM Cell Based ELISA Kit (CB5074)
Western blot analysis of extracts from COS7 cells, using Anti-BIM Antibody.
Protocol Diagram - BIM Cell Based ELISA Kit (CB5074) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: BIM Cell Based ELISA Kit (CB5074)
A diagram showing the protocol for BIM Cell Based ELISA Kit.
Protocol Summary - BIM Cell Based ELISA Kit (CB5074) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: BIM Cell Based ELISA Kit (CB5074)
A summary of the protocol for BIM Cell Based ELISA Kit.
Western Blot - Control Antibody - BIM Cell Based ELISA Kit (CB5074) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - BIM Cell Based ELISA Kit (CB5074)
The mouse monoclonal antibody to GAPDH is used as a positive control inBIM Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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