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Anti-beta Galactosidase Anticorps (A257)

Ce produit est identique à Abcam (ab221199). Découvrez plus pour moins cher.

Western blot analysis of beta galactosidase in E. coli crude extract. Immunoprecipitation of beta galactosidase from E. coli crude extract. Immunofluorescence staining of beta galactosidase expressed in HEK293A cells. HEK293A cells were transfected with beta-Gal cDNA, fixed with 4% paraformaldehyde 24 hrs later, permeabilized with methanol, and immunostained with Anti-beta Galactosidase Antibody (1:500) and Anti-Rabbit IgG (Alexa 555) (1/500). Chromosomal DNA was stained with Hoechst 33342. Scale bar, 50 mm. Note that the antibody only reacts with transfected cells. Titration of antibody reactivity of Anti-beta Galactosidase Antibody by ELISA. Plate was coated with 100ng of beta galactosidase per well and 100µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, Goat Anti-Rabbit IgG (HRP) was added as secondary antibody. Color was developed with TMB as substrate.
Recommandé
pour "beta galactosidase Anticorps"
$370

Anticorps polyclonal de lapin contre beta Galactosidase pour WB, IP, ICC/IF et ELISA.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-beta Galactosidase Antibody
Description du produit
Rabbit polyclonal antibody to beta Galactosidase.
Applications
WB, IP, ICC/IF, ELISA
Recommander des dilutions
WB: 1:1,000-1:2,000, IP: 1:200-1:500, ICC/IF: 1:200-1:500, ELISA: 1:2,000-1:3,000
Reactivité
Escherichia coli beta Galactosidase and beta Gal Tagged proteins.
Immunogène
Full length E. coli beta Galactosidase.
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Protein A chromatography.
Concentration
2 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Acid beta galactosidase, Beta D galactosidase, Beta gal, Beta galactosidase 1, EBP, ECK0341, ELNR1, Galactosidase beta 1, GLB1, JW0335, Lactase, lacZ, MPS4B
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western blot analysis of beta galactosidase in E. coli crude extract.Cliquez pour ouvrir le point de vue élargi
Figure 1: Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody
Immunoprecipitation of beta galactosidase from E. coli crude extract.Cliquez pour ouvrir le point de vue élargi
Figure 2: Immunoprecipitation - Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody
Immunofluorescence staining of beta galactosidase expressed in HEK293A cells. HEK293A cells were transfected with beta-Gal cDNA, fixed with 4% paraformaldehyde 24 hrs later, permeabilized with methanol, and immunostained with Anti-beta Galactosidase Antibody (1:500) and Anti-Rabbit IgG (Alexa 555) (1/500). Chromosomal DNA was stained with Hoechst 33342. Scale bar, 50 mm. Note that the antibody only reacts with transfected cells.Cliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody
Titration of antibody reactivity of Anti-beta Galactosidase Antibody by ELISA. Plate was coated with 100ng of beta galactosidase per well and 100µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, Goat Anti-Rabbit IgG (HRP) was added as secondary antibody. Color was developed with TMB as substrate.Cliquez pour ouvrir le point de vue élargi
Figure 4: Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody

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