Anti-beta Arrestin 1 Antibody (A12755)

Name: Anti-beta Arrestin 1 Antibody
Brand: Antibodies.com
SKU: A12755
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to beta Arrestin 1 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-beta Arrestin 1 Antibody

Description

Rabbit polyclonal antibody to beta Arrestin 1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 169-418 of human ß-arrestin1 (NP_004032.2).

Sequence

RKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQYADICLFNTAQYKCPVAMEEADDTVAPSSTFCKVYTLTPFLANNREKRGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPKEEPPHREVPENETPVDTNLIELDTNDDDIVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

51 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ARR1, ARRB1, Arrestin beta-1, Beta-arrestin-1, Non-visual arrestin-2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-beta Arrestin 1 Antibody (A12755)

Western blot analysis of extracts of various cell lines, using Anti-beta Arrestin 1 Antibody (A12755) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-beta Arrestin 1 Antibody (A12755)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-beta Arrestin 1 Antibody (A12755) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-beta Arrestin 1 Antibody (A12755)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-beta Arrestin 1 Antibody (A12755) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-beta Arrestin 1 Antibody (A12755)

Immunofluorescence analysis of A-549 cells using Anti-beta Arrestin 1 Antibody (A12755) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).