Mouse monoclonal [ACTB/1108] antibody to beta Actin.
Spécificité
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas β-Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
Applications
WB, IHC-P
Recommander des dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivité
Human, Mouse, Rat, Hamster, Monkey, Canine
Immunogène
Recombinant full-length human beta Actin.
Hôte
Mouse
Clonalité
Monoclonal
Clone
ACTB/1108
Isotype
IgG2b
Chaînes légères
kappa
Conjuguer
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Masse moléculaire
43 kDa
Forme du produit
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue using Anti-beta Actin Antibody [ACTB/1108]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human thyroid tissue using Anti-beta Actin Antibody [ACTB/1108]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.
Produits alternatifs à Anti-beta Actin Anticorps [ACTB/1108] (A277773)