Mouse monoclonal [B2M-02] antibody to beta 2 Microglobulin.
Spécificité
This antibody reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Applications
WB, IHC-P, ELISA, Flow Cytometry, ICC
Recommander des dilutions
IHC-P: Heat-mediated antigen retrieval: 5 µg/ml; Positive Control: liver, WB: 2 µg/ml; Non-reducing conditions preferred, Flow Cytometry: 1 µg/ml; Positive Control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes, ELISA: Working dilution should be determinated by investigator.
Reactivité
Porcine, Human
Immunogène
Purified isolated human beta2-microglobulin.
Hôte
Mouse
Clonalité
Monoclonal
Clone
B2M-02
Isotype
IgG1
Conjuguer
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
MW prédit
12 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonymes
B2M, B2MG_HUMAN, Beta 2 microglobin, Beta 2 microglobulin precursor , Beta chain of mhc class 1 proteins , Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, CDABP0092, Hdcma22p, IMD43