Figure 1: Western Blot - Anti-BDNF Antibody (A304943)
Western blot analysis of mouse brain showing detection of ~27.9 Kda BDNF protein using Anti-BDNF Antibody (A304943) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: mouse brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~27.9 Kda.
Figure 2: Western Blot - Anti-BDNF Antibody (A304943)
Western blot analysis of human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Anti-BDNF Antibody (A304943) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human HeLa (20 µg). Lane 3: human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~27.9 Kda.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-BDNF Antibody (A304943), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.
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