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Bak Cell Based Kit Elisa (A103558)

Nom du produit
Bak Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This Bak Cell Based ELISA Kit allows for the detection of Bak and the effects that certain stimulation conditions have on Bak expression in different cell lines. Qualitative determination of Bak concentration is achieved by an indirect ELISA format. In essence, the Bak is captured by Anti-Bak Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Bak Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Apoptosis regulator BAK, BAK like, Bak NT, Bak1, BAK_HUMAN, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, BCL2 antagonist/killer 1, Bcl2 like 7 Protein, Bcl2-L-7, BCL2L7, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Bak Cell Based ELISA Kit (CB5065) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Bak Cell Based ELISA Kit (CB5065)
Western blot analysis of extracts from 293 cells, using Anti-Bak Antibody.
Protocol Diagram - Bak Cell Based ELISA Kit (CB5065) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Bak Cell Based ELISA Kit (CB5065)
A diagram showing the protocol for Bak Cell Based ELISA Kit.
Protocol Summary - Bak Cell Based ELISA Kit (CB5065) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Bak Cell Based ELISA Kit (CB5065)
A summary of the protocol for Bak Cell Based ELISA Kit.
Western Blot - Control Antibody - Bak Cell Based ELISA Kit (CB5065) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - Bak Cell Based ELISA Kit (CB5065)
The mouse monoclonal antibody to GAPDH is used as a positive control inBak Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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