Rabbit polyclonal antibody to BAD (Phospho-Ser136)
Specificity
The antibody detects endogenous level of BAD only when phosphorylated at serine136.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine136 (S-R-S(p)-A-P) derived from Mouse BAD.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
23kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
BBC6, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-associated agonist of cell death, Bcl2-L-8, BCL2L8
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136) Antibody #11068 (left) or the same antibody preincubated with blocking peptide (right).
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