Anti-Aurora B Kinase Anticorps [3F11] (A85381)

Name: Anti-Aurora B Kinase Antibody [3F11]
Brand: Antibodies.com
SKU: A85381
Price: 185.0 USD
Availability: InStock

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$185

Anticorps monoclonal [3F11] de souris contre Aurora B pour WB, ICC/IF et IHC.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Spécifications

Nom du produit

Anti-Aurora B Kinase Antibody [3F11]

Description du produit

Mouse monoclonal (3F11) antibody to Aurora B Kinase.

Spécificité

This antibody is specific for Aurora B and does not cross-react with Aurora A and Aurora C. This antibody can be used to identify dividing or soon to be dividing cells and the antibody is also an excellent marker of midbodies both during and after cell division.

Applications

WB, ICC/IF, IHC

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:1,000, IHC: 1:1,000

Reactivité

Human, Horse, Bovine, Rat, Mouse

Immunogène

Recombinant full-length human Aurora B protein, expressed in and purified from E. coli.

Hôte

Mouse

Clonalité

Monoclonal

Clone

3F11

Isotype

IgG2a

Conjuguer

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Masse moléculaire

38 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, AURKB, AURKB_HUMAN, Aurora 1, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora related kinase 2, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1 related kinase 2, Aurora/IPL1-related kinase 2, IPL1, PPP1R48, Protein phosphatase 1 regulatory subunit 48, Serine/theronine kinase 12, Serine/threonine protein kinase 12, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase aurora-B, STK-1, STK1, STK12, STK5

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Immunofluorescence - Anti-Aurora B Kinase Antibody [3F11] (A85381)

High magnification confocal immunofluorescence of HeLa cells stained with Anti-Aurora B Kinase Antibody (1:1,000 | green) and Anti-Vimentin Antibody (A85421 | 1:1,000 | red). Blue is the DNA stain DAPI revealing the nuclei. The Anti-Aurora B Kinase Antibody reveals aurora B localized in midbodies, midzones of dividing cells and also in the nuclei or some cells.

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Figure 2: Immunofluorescence - Anti-Aurora B Kinase Antibody [3F11] (A85381)

HeLa cell cultures were stained with Anti-Aurora B Kinase Antibody (green). Anti-Aurora B Kinase Antibody stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with Anti-Vimentin Antibody (A85421 | red). Blue is a DNA stain.

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Figure 3: Western Blot - Anti-Aurora B Kinase Antibody [3F11] (A85381)

Western blot analysis of different cell lysates and recombinant protein solutions using Anti-Aurora B Kinase Antibody [3F11] (A85381), in green. Left: Cells were treated with 100 ng/ml of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. The lanes contain samples of: [1] Protein standards, in red, [2] HeLa cells, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Western blot of purified full-length recombinant human Aurora A, B and C, probed with Anti-Aurora B Kinase Antibody [3F11] (A85381). The antibody binds specifically only to Aurora B and not to the closely related Aurora A and C, shown by the single band in lane B.

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Figure 4: Western Blot - Anti-Aurora B Kinase Antibody [3F11] (A85381)

Left: Western blot analysis of Anti-Aurora B Kinase Antibody on lysateas from HeLa cells treated with 100ng/ml nocodazole for 18 hours. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. The Anti-Aurora B Kinase Antibody binds strongly to the aurora B band at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with Anti-Aurora B Kinase Antibody. This antibody binds specifically only to aurora B and not to the closely related aurora A and C. This is also consistent with the immunocytochemical staining of midzones and midbodies on HeLa cells.