Synthetic peptide within amino acids 2550-2600 of human ATM.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Sample: FFPE section of human prostate-nodular hypertrophy. Antibody: Anti-ATM Antibody (A295418) was used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB.
Samples: Whole cell lysate (1 mg) from WT cells. Antibody: Anti-ATM Antibody (A295418) was used at 0.5 µg/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds.
Samples: Whole cell lysate (50 µg) from human L-40 (WT) or AT-59 (AT) cells. Antibody: Anti-ATM Antibody (A295418) was used at the indicated concentrations for WB. Detection: Chemiluminescence with an exposure time of 30 seconds.
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