Anti-ATG4D Anticorps (A304858)

Name: Anti-ATG4D Antibody
Brand: Antibodies.com
SKU: A304858
Price: 500.0 USD
Availability: InStock

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$500

Anticorps polyclonal de lapin contre ATG4D pour WB et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-ATG4D Antibody

Description du produit

Rabbit polyclonal antibody to ATG4D.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivité

Human

Immunogène

Synthetic peptide from the N-terminal of Human ATG4D (aa. 98-108).

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Masse moléculaire

~53 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

9830134P12Rik, APG4 autophagy 4 homolog D, APG4 D, APG4, S. cerevisiae, homolog of, D, APG4D, ATG4 autophagy related 4 homolog D (S. cerevisiae), Atg4dl, ATG4D_HUMAN, AUT like 4 cysteine endopeptidase, AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin 4, Autophagin-4, autophagy 4, S. cerevisiae, homolog of, D, autophagy related 4D, cysteine peptidase, Autophagy related cysteine endopeptidase 4, Autophagy related protein 4 homolog D, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, Cysteine protease involved in autophagy, MGC31226

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-ATG4D Antibody (A304858)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Anti-ATG4D Antibody (A304858) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: human HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.

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Figure 2: Immunofluorescence - Anti-ATG4D Antibody (A304858)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG4D Antibody (A304858), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4D Antibody. (D) Composite.