Anti-ATG12 Anticorps (A90451)

Western blot analysis of extracts of various cell lines, using Anti-ATG12 Antibody (A90451) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Immunofluorescence analysis of NIH/3T3 cells using Anti-ATG12 Antibody (A90451) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of PC-12 cells using Anti-ATG12 Antibody (A90451) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of U2OS cells using Anti-ATG12 Antibody (A90451) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).