Anti-ATF6 Anticorps (A85123)

Name: Anti-ATF6 Antibody
Brand: Antibodies.com
SKU: A85123
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab174756). Découvrez plus pour moins cher.

Cliquez pour ouvrir le point de vue élargi

$480

Anticorps polyclonal de chèvre contre ATF6 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-ATF6 Antibody

Description du produit

Goat polyclonal antibody to ATF6.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:128,000, WB: 0.3-2µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region, amino acids 437-449, of human ATF6.

Séquence

C-NSYRYDHSVSNDK

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

75-80 kDa

MW prédit

74.6kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Activating transcription factor 6, Activating transcription factor 6 alpha, ATF 6, ATF6 alpha, ATF6-alpha, ATF6A, ATF6A_HUMAN, cAMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Cyclic AMP dependent transcription factor ATF 6 alpha, DKFZp686P2194, ESTM49, FLJ21663, Processed cyclic AMP dependent transcription factor ATF 6 alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-ATF6 Antibody (A85123)

ATF6 expression in HeLa (A), MCF7 (B), HeLa nuclear (C), and Jurkat nuclear (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ATF6 Antibody (A85123) at 1µg/ml and detected by chemiluminescence.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunofluorescence - Anti-ATF6 Antibody (A85123)

ATF6 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunofluorescence - Anti-ATF6 Antibody (A85123)

ATF6 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Flow Cytometry - Anti-ATF6 Antibody (A85123)

ATF6 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.