This antibody recognises human ASK1, also known as apoptosis signal-regulating kinase 1, MAP kinase kinase kinase or MAPKKK. ASK1 is a ~155 kDa protein belonging to the protein kinase superfamily.ASK1 is a serine/threonine kinase and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.ASK1 is widely expressed in a variety of human tissues including the heart and pancreas.
Applications
WB, IHC-Fr
Dilutions
IHC-Fr: 10 µg/ml, WB: 0.5 - 1 µg/ml
Reactivity
Human
Immunogen
A peptide corresponding to amino acids near the C-terminus of human ASK1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
500 µg/ml
Molecular Weight
Approximately 155 kDa in SW1353 cell lysates.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Human ASK1 antibody recognises human ASK1, also known as apoptosis signal-regulating kinase 1, MAP kinase kinase kinase or MAPKKK. ASK1 is a ~155 kDa protein belonging to the protein kinase superfamily. ASK1 is a serine/threonine kinase and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis. ASK1 is widely expressed in a variety of human tissues including the heart and pancreas.
