Figure 1: Western Blot - Anti-ARSA/ASA Antibody (A84257)
ARSA/ASA expression in Mouse Testes lysate (A) + peptide (B) and Rat Testes lysate (C) + peptide (D) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-ARSA/ASA Antibody (A84257) at 1µg/ml (A-B) or 0.3µg/ml (C-D) and detected by chemiluminescence.
ARSA/ASA expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ARSA/ASA Antibody (A84257) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
ARSA/ASA expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ARSA/ASA Antibody (A84257) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
ARSA/ASA expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ARSA/ASA Antibody (A84257) at 5µg/ml and revealed with alkaline phosphatase (AP).
ARSA/ASA expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ARSA/ASA Antibody (A84257) at 5µg/ml and revealed with alkaline phosphatase (AP).
