Anti-ARF1 Anticorps (A83143)

Name: Anti-ARF1 Antibody
Brand: Antibodies.com
SKU: A83143
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab58578). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre ARF1 pour ELISA, WB et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-ARF1 Antibody

Description du produit

Goat polyclonal antibody to ARF1.

Applications

ELISA, WB, Flow Cytometry

Recommander des dilutions

ELISA: 1:8,000, WB: 0.3-1µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human, Mouse, Rat, Canine

Immunogène

Synthetic peptide corresponding to the C terminal region of human ARF1.

Séquence

C-EGLDWLSNQLRNQK

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

18 kDa

MW prédit

20.7kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

ADP Ribosylation Factor 1, ADP-ribosylation factor 1, ARF 1, ARF1_HUMAN

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-ARF1 Antibody (A83143)

ARF1 expression in A431 (A) and HeLa (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody was performed with Anti-ARF1 Antibody (A83143) at 1µg/ml (A) or 0.3µg/ml (B) and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-ARF1 Antibody (A83143)

ARF1 expression in NIH3T3 (A), KNRK (B) and MDCK (C) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ARF1 Antibody (A83143) at 1µg/ml and detected by chemiluminescence.

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Figure 3: Flow Cytometry - Anti-ARF1 Antibody (A83143)

ARF1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ARF1 Antibody (A83143) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.