Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ADH water channel, AQP-2, AQP-CD, AQP2, Aquaporin-2, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct, WCH-CD
Western blot analysis of extracts of 293T cells, using Anti-Aquaporin 2 Antibody (A89116) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Aquaporin 2 Antibody (A89116) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat kidney cells using Anti-Aquaporin 2 Antibody (A89116) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse kidney cells using Anti-Aquaporin 2 Antibody (A89116) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Aquaporin 2 Antibody (A89116)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-Aquaporin 2 Antibody (A89116)
