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Anti-APP-1 Antibody (A15060)

Western Blot - Anti-APP-1 Antibody (A15060) - Antibodies.com Immunohistochemistry - Anti-APP-1 Antibody (A15060) - Antibodies.com Immunohistochemistry - Anti-APP-1 Antibody (A15060) - Antibodies.com Immunofluorescence - Anti-APP-1 Antibody (A15060) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to APP-1 for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-APP-1 Antibody
Description
Rabbit polyclonal antibody to APP-1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).
Sequence
VTEMNGRIVGSKPLYVALAQRKEERKAHLTNQYMQRVAGMRALPANAILNQFQPAAGGYFVPAVPQAQGRPPYYTPNQLAQMRPNPRWQQGGRPQGFQGMP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
79 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Activated-platelet protein 1, APP1, Inducible poly(A)-binding protein, iPABP, PABP-4, PABP4, Poly(A)-binding protein 4, Polyadenylate-binding protein 4
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-APP-1 Antibody (A15060) - Antibodies.comEnlarge Image

Western Blot - Anti-APP-1 Antibody (A15060)

Western blot analysis of extracts of various cell lines, using Anti-APP-1 Antibody (A15060) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-APP-1 Antibody (A15060) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-APP-1 Antibody (A15060)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-APP-1 Antibody (A15060) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-APP-1 Antibody (A15060) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-APP-1 Antibody (A15060)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-APP-1 Antibody (A15060) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-APP-1 Antibody (A15060) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-APP-1 Antibody (A15060)

Immunofluorescence analysis of U2OS cells using Anti-APP-1 Antibody (A15060) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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