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APEX1 Cell Based Kit Elisa (A103035)

Nom du produit
APEX1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This APEX1 Cell Based ELISA Kit allows for the detection of APEX1 and the effects that certain stimulation conditions have on APEX1 expression in different cell lines. Qualitative determination of APEX1 concentration is achieved by an indirect ELISA format. In essence, the APEX1 is captured by Anti-APEX1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this APEX1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
AP endonuclease 1, AP endonuclease class I, AP lyase, APE, APE 1, APE-1, APE1, APEN, APEX, APEX 1, APEX nuclease, APEX nuclease (multifunctional DNA repair enzyme) 1, Apex nuclease 1, APEX1_HUMAN, Apurinic endonuclease, Apurinic-apyrimidinic endonuclease 1, Apurinic/apyrimidinic (abasic) endonuclease, Apurinic/apyrimidinic endonuclease 1, Apurinic/apyrimidinic exonuclease, APX, BAP1, Deoxyribonuclease (apurinic or apyrimidinic), DNA (apurinic or apyrimidinic site) lyase, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, EC 4.2.99.18, HAP 1, HAP1, Human Apurinic endonuclease 1, MGC139790, Multifunctional DNA repair enzyme, Redox factor 1, Redox factor-1, REF 1, REF 1 protein, REF-1, REF1, REF1 protein
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - APEX1 Cell Based ELISA Kit (CB5867) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - APEX1 Cell Based ELISA Kit (CB5867)
Western blot analysis of extracts from COLO205/HeLa cells, using Anti-APEX1 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - APEX1 Cell Based ELISA Kit (CB5867) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: APEX1 Cell Based ELISA Kit (CB5867)
A diagram showing the protocol for APEX1 Cell Based ELISA Kit.
Protocol Summary - APEX1 Cell Based ELISA Kit (CB5867) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: APEX1 Cell Based ELISA Kit (CB5867)
A summary of the protocol for APEX1 Cell Based ELISA Kit.
Western Blot - Control Antibody - APEX1 Cell Based ELISA Kit (CB5867) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - APEX1 Cell Based ELISA Kit (CB5867)
The mouse monoclonal antibody to GAPDH is used as a positive control inAPEX1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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