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AOS1 Cell Based Kit Elisa (A103215)

Nom du produit
AOS1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This AOS1 Cell Based ELISA Kit allows for the detection of AOS1 and the effects that certain stimulation conditions have on AOS1 expression in different cell lines. Qualitative determination of AOS1 concentration is achieved by an indirect ELISA format. In essence, the AOS1 is captured by Anti-AOS1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this AOS1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Activator of SUMO1, HSPC140, SAE1, SAE1_HUMAN, Sentrin/SUMO activating protein AOS1, SUA1, SUMO 1 activating enzyme E1 N subunit, SUMO 1 activating enzyme subunit 1, SUMO-activating enzyme subunit 1, Ubiquitin like protein SUMO1 activating enzyme, Ubiquitin-like 1-activating enzyme E1A, UBL E1A, UBLE1A
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - AOS1 Cell Based ELISA Kit (CB5042) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - AOS1 Cell Based ELISA Kit (CB5042)
Western blot analysis of extracts from 293 cells, using Anti-AOS1 Antibody.
Protocol Diagram - AOS1 Cell Based ELISA Kit (CB5042) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: AOS1 Cell Based ELISA Kit (CB5042)
A diagram showing the protocol for AOS1 Cell Based ELISA Kit.
Protocol Summary - AOS1 Cell Based ELISA Kit (CB5042) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: AOS1 Cell Based ELISA Kit (CB5042)
A summary of the protocol for AOS1 Cell Based ELISA Kit.
Western Blot - Control Antibody - AOS1 Cell Based ELISA Kit (CB5042) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - AOS1 Cell Based ELISA Kit (CB5042)
The mouse monoclonal antibody to GAPDH is used as a positive control inAOS1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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