Nous Contacter
+1 (314) 370-6046 ouNous Contacter
Visit our English Website
Antibodies.com LogoAntibodies.com Logo
Se connecter / S'inscrireMon Compte
0

ANKRD1 Cell Based Kit Elisa (A103027)

Nom du produit
ANKRD1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This ANKRD1 Cell Based ELISA Kit allows for the detection of ANKRD1 and the effects that certain stimulation conditions have on ANKRD1 expression in different cell lines. Qualitative determination of ANKRD1 concentration is achieved by an indirect ELISA format. In essence, the ANKRD1 is captured by Anti-ANKRD1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this ANKRD1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
ALRP, ANKR1_HUMAN, ANKRD 1, Ankyrin repeat domain 1 (cardiac muscle), Ankyrin repeat domain containing protein 1, Ankyrin repeat domain-containing protein 1, bA320F15.2, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, Cytokine inducible nuclear protein, Cytokine-inducible gene C-193 protein, Cytokine-inducible nuclear protein, HA1A2, Liver ankyrin repeat domain 1, MCARP, OTTHUMP00000020084
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - ANKRD1 Cell Based ELISA Kit (CB6017) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - ANKRD1 Cell Based ELISA Kit (CB6017)
Western blot analysis of extracts from COLO205 cells, using Anti-ANKRD1 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - ANKRD1 Cell Based ELISA Kit (CB6017) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: ANKRD1 Cell Based ELISA Kit (CB6017)
A diagram showing the protocol for ANKRD1 Cell Based ELISA Kit.
Protocol Summary - ANKRD1 Cell Based ELISA Kit (CB6017) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: ANKRD1 Cell Based ELISA Kit (CB6017)
A summary of the protocol for ANKRD1 Cell Based ELISA Kit.
Western Blot - Control Antibody - ANKRD1 Cell Based ELISA Kit (CB6017) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - ANKRD1 Cell Based ELISA Kit (CB6017)
The mouse monoclonal antibody to GAPDH is used as a positive control inANKRD1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Menu Principal Nous Contacter 0Caisse
Haut