Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein (Biotin).
Spécificité
This antibody reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
Applications
WB, ICC, ELISA, IP
Recommander des dilutions
ELISA: This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha fetoprotein in combination with A85534, WB: 1-2 µg/ml; Non-reducing conditions, IP: Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
Reactivité
Human
Immunogène
Purified alpha Fetoprotein.
Hôte
Mouse
Clonalité
Monoclonal
Clone
AFP-01
Isotype
IgG1
Conjuguer
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
MW prédit
70 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.