Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, CRYAB, Heat shock protein beta-5, Heat shock protein family B member 5, HSPB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Western Blot - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)
Western blot analysis of extracts of various cell lines, using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)
Immunofluorescence analysis of C6 cells using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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