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Anti-Alpha B Crystallin Anticorps [ARC1672] (A308835)

Western Blot - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) - Antibodies.com Immunohistochemistry - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) - Antibodies.com Immunofluorescence - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) - Antibodies.com
$470
Product Datasheet

Anticorps monoclonal [ARC1672] de lapin contre Alpha B Crystallin pour WB, IHC et ICC/IF.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Alpha B Crystallin Antibody [ARC1672]
Description du produit
Rabbit monoclonal [ARC1672] antibody to Alpha B Crystallin.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 76-175 of human CRYAB (P02511).
Séquence
SVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC1672
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
20 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
AACRYA, Alpha crystallin B chain, Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, Cryab, CRYAB_HUMAN, Crystallin alpha B, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY REN 27, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)
Western blot analysis of extracts of various cell lines, using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)
Immunofluorescence analysis of C6 cells using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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