Figure 1: Western Blot - Anti-AKT1 Antibody [ARC51585] (A308667)
Western blot analysis of various lysates, using Anti-AKT1 Antibody [ARC51585] (A308667) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 25s.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 7: Western Blot - Anti-AKT1 Antibody [ARC51585] (A308667)
Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-AKT1 Antibody [ARC51585] (A308667). This Western blot was performed on the immunoprecipitate using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:1000.