Anti-AKT1 Anticorps (A285973)

Name: Anti-AKT1 Antibody
Brand: Antibodies.com
SKU: A285973
Price: 480.0 USD
Availability: InStock

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$480

Anticorps polyclonal de chèvre contre AKT1 pour ELISA, WB et IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-AKT1 Antibody

Description du produit

Goat polyclonal antibody to AKT1.

Applications

ELISA, WB, IF

Recommander des dilutions

ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IF: 10µg/ml

Reactivité

Human

Réactivité croisée

This antibody is not expected to cross react with AKT2 but may recognize AKT1 in Rat and Canine.

Immunogène

Synthetic peptide corresponding to the internal region of human AKT1.

Séquence

C-QDVDQREAPLN

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

60 kDa

MW prédit

55.7kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Western Blot - Anti-AKT1 Antibody (A285973)

AKT1 expression in MCF7 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AKT1 Antibody (A285973) at 0.3µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-AKT1 Antibody (A285973)

AKT1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-AKT1 Antibody (A285973) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/microtubule staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.