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AKAP5 Cell Based Kit Elisa (A103692)

Nom du produit
AKAP5 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This AKAP5 Cell Based ELISA Kit allows for the detection of AKAP5 and the effects that certain stimulation conditions have on AKAP5 expression in different cell lines. Qualitative determination of AKAP5 concentration is achieved by an indirect ELISA format. In essence, the AKAP5 is captured by Anti-AKAP5 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this AKAP5 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
A kinase (PRKA) anchor protein 5, A kinase anchor protein 5, A kinase anchor protein 79kDa, A kinase anchoring protein 75/79, A-kinase anchor protein 5, A-kinase anchor protein 79 kDa, AKAP 5, AKAP 75, AKAP 79, AKAP-5, AKAP5_HUMAN, AKAP75, AKAP79, cAMP dependent protein kinase regulatory subunit II high affinity binding protein, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein, H21
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - AKAP5 Cell Based ELISA Kit (CB5862) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - AKAP5 Cell Based ELISA Kit (CB5862)
Western blot analysis of extracts from HepG2/HeLa/COLO205 cells, using Anti-AKAP5 Antibody.
Protocol Diagram - AKAP5 Cell Based ELISA Kit (CB5862) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: AKAP5 Cell Based ELISA Kit (CB5862)
A diagram showing the protocol for AKAP5 Cell Based ELISA Kit.
Protocol Summary - AKAP5 Cell Based ELISA Kit (CB5862) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: AKAP5 Cell Based ELISA Kit (CB5862)
A summary of the protocol for AKAP5 Cell Based ELISA Kit.
Western Blot - Control Antibody - AKAP5 Cell Based ELISA Kit (CB5862) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - AKAP5 Cell Based ELISA Kit (CB5862)
The mouse monoclonal antibody to GAPDH is used as a positive control inAKAP5 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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