Recombinant human monoclonal [3F6-9G5] antibody to Adenosine Receptor A2a.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivité
Human
Hôte
Human
Clonalité
Monoclonal
Clone
3F6-9G5
Isotype
IgG1
Chaînes légères
kappa
Conjuguer
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Masse moléculaire
This antibody has a predicted MW of 146.66 kDa.
Pureté
> 95% (by SDS-PAGE and SEC-HPLC).
Forme du produit
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Stockage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) confirms that the purity of this antibody is 98.85%.
Human A2AR HEK293 Cell Line were stained with Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) and negative control protein respectively, washed and then followed by PE and analyzed with FACS.
THP-1 were stained with Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, the EC50 was approximately 0.2161 µg/ml.
Cynomolgus/rhesus macaque A2AR HEK293 Cell Line were stained with Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, the EC50 was approximately 0.215 µg/ml.
![SDS-PAGE - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_1.jpg?profile=product_top)
![SEC-HPLC - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_2.jpg?profile=product_top)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_3.jpg?profile=product_top)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_4.jpg?profile=product_top)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_5.jpg?profile=product_top)
![SDS-PAGE - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_1.jpg?profile=product_top_thumb)
![SEC-HPLC - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_2.jpg?profile=product_top_thumb)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_3.jpg?profile=product_top_thumb)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_4.jpg?profile=product_top_thumb)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_5.jpg?profile=product_top_thumb)
![SDS-PAGE - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_1.jpg?profile=product_image)
![SEC-HPLC - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_2.jpg?profile=product_image)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_3.jpg?profile=product_image)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_4.jpg?profile=product_image)
![FACS - Anti-Adenosine Receptor A2a Antibody [3F6-9G5] - Low endotoxin, Azide free (A323879) - Antibodies.com](https://cdn.antibodies.com/image/catalog/323/A323879_5.jpg?profile=product_image)
