Acetyl Coenzyme A Assay Kit (A319654)

$535
Frais de livraison
Date de livraison
Livraison sous 7-8 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Acetyl Coenzyme A Assay Kit
Description du produit
This Acetyl Coenzyme A Assay Kit is a quantitative assay kit for measuring Acetyl-CoA in various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension).
Applications
Functional Studies
Principe du test
This kit provides a simple, sensitive, rapid colorimetric acetyl-CoA detection method. Suitable for various types of samples, especially animals or plant tissues or cells. The detection principle is that malate dehydrogenase can catalyze the reaction of malate acid and NAD+ to produce oxaloacetic acid and NADH. Citrate synthase catalyzes the reaction of acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, acetyl-CoA content is proportional to the generation of NADH. NADH has an absorption peak at 340 nm, and the measurement of the absorbance value of 340 nm enables calculation of the content of acetyl-CoA.
Type d'échantillon
Various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension).
Composants
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Standard
Stockage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Notes générales
96-well UV microplate / microquartz cuvette is required.
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
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