Rabbit polyclonal antibody to 4E-BP1 (phospho Thr45).
Spécificité
This antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr45.
Applications
WB, IHC, ELISA
Recommander des dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000
Reactivité
Human, Mouse, Rat
Immunogène
Synthetic peptide derived from human 4E-BP1 around the phosphorylation site of Thr45 (amino acids 13-62).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Masse moléculaire
12kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-4E-BP1 (phospho Thr45) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Figure 2: Western Blot - Anti-4E-BP1 (phospho Thr45) Antibody (A94219)
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 5' using Anti-4E-BP1 (phospho Thr45) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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