Rabbit polyclonal antibody to 4E-BP1 (Phospho-Thr37)
Specificity
The antibody detects endogenous level of 4E-BP1 only when phosphorylated at threonine 36.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 37(S-T-T(p)-P-G) derived from Human 4E-BP1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C
Synonyms
eIF4E-binding protein 1, EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-37) antibody (#21215, Lane 1 and 2) and 4E-BP1 (phospho-Thr37) antibody (#11222, Lane 3 and 4).
