Rabbit polyclonal antibody to 14-3-3 theta (phospho Ser232).
Spécificité
This antibody detects endogenous levels of 14-3-3 thet/tau only when phosphorylated at Ser232.
Applications
WB, IHC, IF, ELISA
Recommander des dilutions
WB: 1:500-1:1000, ELISA: 1:40000
Reactivité
Human, Mouse
Immunogène
Synthetic peptide derived from human 14-3-3 thet/tau around the phosphorylation site of Ser232 (amino acids 196-245).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Masse moléculaire
27kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
14-3-3 protein T cell, 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 protein theta, 1433T_HUMAN, IC5, Protein HS1, Protein tau, Ywhaq, YWHAQ tyrosine 3 monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Figure 1: Western Blot - Anti-14-3-3 theta (phospho Ser232) Antibody (A94789)
Western blot analysis of lysates from HeLa cells using Anti-14-3-3 theta (phospho Ser232) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human pancreas using Anti-14-3-3 theta (phospho Ser232) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-14-3-3 theta (phospho Ser232) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
