Anti-FOXP3 Antibody (A82808)

FOXP3 expression in Human Muscle (A), MOLT4 (B), and negative control Pancreas (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-FOXP3 Antibody (A82808) at 1µg/ml (A), 2µg/ml (B), or 1µg/ml (C) and detected by chemiluminescence.

FOXP3 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-FOXP3 Antibody (A82808) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Strong localization to nucleoplasm shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

FOXP3 expression in Jurkat cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-FOXP3 Antibody (A82808) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

FOXP3 expression in CD25-sorted (Treg) Human blood cells gathered by cytospin analyzed by immunofluorescence. Staining was performed with Anti-FOXP3 Antibody (A82808) at 5µg/ml and detected by FITC (A) and in phase contrast (B).