Anti-FOXO3A (phospho Ser425) Antibody (A306586)

Western blot analysis of extracts of K-562 cells, using Anti-FOXO3A (phospho Ser425) Antibody (A306586) at 1:1,000 dilution. K-562 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-FOXO3A (phospho Ser425) Antibody (A306586) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-FOXO3A (phospho Ser425) Antibody (A306586) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-FOXO3A (phospho Ser425) Antibody (A306586) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.