Anti-FOXO3A (phospho Ser253) Antibody (A91783)

Name: Anti-FOXO3A (phospho Ser253) Antibody
Brand: Antibodies.com
SKU: A91783
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to FOXO3A (phospho Ser253) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-FOXO3A (phospho Ser253) Antibody

Description

Rabbit polyclonal antibody to FOXO3A (phospho Ser253).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S253 of human FOXO3A (NP_001446.1).

Sequence

AVSMD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

97 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AF6q21 protein, FKHRL1, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1, FOXO3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-FOXO3A (phospho Ser253) Antibody (A91783)

Western blot analysis of extracts of 293T cells, using Anti-FOXO3A (phospho Ser253) Antibody (A91783) at 1:2,000 dilution. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-FOXO3A (phospho Ser253) Antibody (A91783)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-FOXO3A (phospho Ser253) Antibody (A91783) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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