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Anti-FOXC2 Antibody (A83941)

Western Blot - Anti-FOXC2 Antibody (A83941) - Antibodies.com Flow Cytometry - Anti-FOXC2 Antibody (A83941) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to FOXC2 for ELISA, WB and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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+1 (314) 370-6046
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Name
Anti-FOXC2 Antibody
Description
Goat polyclonal antibody to FOXC2.
Applications
ELISA, WB, Flow Cytometry
Dilutions
ELISA: 1:4,000, WB: 0.3-1µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human FOXC2.
Sequence
RHAAPYSYDCTKY
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
60-70 kDa
Predicted MW
53.7kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Drosphilia Forkhead Homolog Like 14, FKHL 14, FKHL14, Forkhead Box C2, Forkhead box protein C2, Forkhead related protein FKHL14, Forkhead-related protein FKHL14, FOX C2, FOXC2_HUMAN, LD, Mesenchyme fork head protein 1, Mesenchyme Forkhead 1, MFH 1, MFH 1 protein, MFH-1 protein, MFH1, Transcription factor FKH 14, Transcription factor FKH-14
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-FOXC2 Antibody (A83941) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-FOXC2 Antibody (A83941)
FOXC2 expression in HEK293 nuclear and negative control Human Pancreas (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody was performed with Anti-FOXC2 Antibody (A83941) at 2µg/ml and detected by chemiluminescence.
Flow Cytometry - Anti-FOXC2 Antibody (A83941) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-FOXC2 Antibody (A83941)
FOXC2 expression in HEK293 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-FOXC2 Antibody (A83941) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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