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Anti-Flotillin 1 Antibody (Biotin) (A82833)

Western Blot - Anti-FLOT1 Antibody (A82833) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to Flotillin 1 (Biotin) for ELISA and WB.

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$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-Flotillin 1 Antibody (Biotin)
Description
Goat polyclonal antibody to Flotillin 1 (Biotin).
Applications
ELISA, WB
Dilutions
ELISA: 1:2,000, WB: 1-3µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human Flotillin 1.
Sequence
CSISQVNHKPLRTA.
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Biotin

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
48 kDa
Predicted MW
47.4kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
FLOT 1, FLOT1, FLOT1_HUMAN, Flotillin-1, Flotillin1, Integral membrane component of caveolae, Reggie 2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-FLOT1 Antibody (A82833) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Flotillin 1 Antibody (Biotin) (A82833)
Anti-FLOT1 Antibody (A82833 | Biotin) (1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
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