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Anti-Flotillin 1 Antibody (A82834)
This product is identical to Abcam (ab13493). Discover more for less.
Overview
Specifications
Images
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$480
Product Datasheet
Goat polyclonal antibody to Flotillin 1 for ELISA, WB and IHC.
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Product Size:
100µg
Quantity:
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Alternative Formats and Conjugates
Alternative Formats
Biotin
Shipping Information
Delivery
Freight Charges
$40 to United States
Lead Time
3-5 Business Days
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Specifications
Name
Anti-Flotillin 1 Antibody
Description
Goat polyclonal antibody to Flotillin 1.
Applications
ELISA
,
WB
,
IHC
Dilutions
ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IHC: 5µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human Flotillin 1.
Sequence
C-SISQVNHKPLRTA
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
48 kDa
Predicted MW
47.4kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
FLOT 1, FLOT1, FLOT1_HUMAN, Flotillin-1, Flotillin1, Integral membrane component of caveolae, Reggie 2
Isotype Controls
Goat IgG (A121671)
Suitable Secondaries
Donkey Anti-Goat IgG H&L Antibody (AP) (A300679)
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A300716)
Donkey Anti-Goat IgG H&L Antibody (FITC) (A300685)
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300730)
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (3)
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Figure 1: Immunohistochemistry - Anti-Flotillin 1 Antibody (A82834)
Anti-FLOT1 Antibody (A82834) (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Figure 2: Western Blot - Anti-Flotillin 1 Antibody (A82834)
Anti-FLOT1 Antibody (A82834) staining (0.3µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Enlarge Image
Figure 3: Western Blot - Anti-Flotillin 1 Antibody (A82834)
HEK293 overexpressing FLOT1(RC200231) and probed with Anti-FLOT1 Antibody (A82834) (mock transfection in first lane), tested by Origene.
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