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FLI1 Cell Based ELISA Kit (A103508)

Western Blot - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.com Protocol Diagram - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.com Protocol Summary - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.com Western Blot - Control Antibody - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
FLI1 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This FLI1 Cell Based ELISA Kit allows for the detection of FLI1 and the effects that certain stimulation conditions have on FLI1 expression in different cell lines. Qualitative determination of FLI1 concentration is achieved by an indirect ELISA format. In essence, the FLI1 is captured by Anti-FLI1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this FLI1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
ERGB transcription factor, Ewing Sarcoma breakpoint region 2, EWSR 2, EWSR2, FLI 1, FLI 1 proto oncogene, Fli-1 proto-oncogene, ETS transcription factor, FLI1 EWS fusion gene, FLI1 proto oncogene, FLI1_HUMAN, Friend leukemia integration 1 (FLI1) transcription factor, Friend leukemia integration 1 transcription factor, Friend leukemia virus integration 1, Proto-oncogene Fli-1, Sarcoma breakpoint region 2 (EWSR2), SIC 1, SIC1, Transcription factor ERGB, Viral integration region FLI1, Viral integration region FLI1, mouse, homolog of
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.comEnlarge Image
Figure 1: Western Blot - FLI1 Cell Based ELISA Kit (A103508)
Western blot analysis of extracts from NIH-3T3 cells, using Anti-FLI1 Antibody.
Protocol Diagram - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.comEnlarge Image
Figure 2: FLI1 Cell Based ELISA Kit (A103508)
A diagram showing the protocol for FLI1 Cell Based ELISA Kit.
Protocol Summary - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.comEnlarge Image
Figure 3: FLI1 Cell Based ELISA Kit (A103508)
A summary of the protocol for FLI1 Cell Based ELISA Kit.
Western Blot - Control Antibody - FLI1 Cell Based ELISA Kit (CB5270) - Antibodies.comEnlarge Image
Figure 4: Western Blot - FLI1 Cell Based ELISA Kit (A103508)
The mouse monoclonal antibody to GAPDH is used as a positive control inFLI1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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