Anti-FLI1 Antibody [FLI1/7508] (A314971)

Anti-FLI1 Antibody [FLI1/7508]

Mouse monoclonal [FLI1/7508] antibody to FLI1.

Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing s Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

WB, IHC-P

THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma

WB: 1-2 µg/ml, IHC: 1-2 µg/ml

Human

Recombinant fragment (aa 107-261) of human FLI1 protein with hexa-histidine tag.

Mouse

Monoclonal

IgG2

kappa

Protein A chromatography.

200 µg/ml

51 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB