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Anti-Flag-Tag Antibody (A50042)

Flag-Tag Mouse Monoclonal Antibody from Signalway Antibody (T519) - Antibodies.com Flag-Tag Mouse Monoclonal Antibody from Signalway Antibody (T519) - Antibodies.com Flag-Tag Mouse Monoclonal Antibody from Signalway Antibody (T519) - Antibodies.com
$390

Mouse monoclonal antibody to Flag-Tag for WB, IF and IP.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Flag-Tag Antibody
Description
Mouse monoclonal antibody to Flag-Tag
Specificity
The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.
Applications
WB, IF, IP
Reactivity
Human, Mouse, Rat
Host
Mouse
Clonality
Monoclonal
Conjugate

Unconjugated

Purification
Affinity purification using immunogen.
Concentration
1.0mg / ml
Formulation
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flag-Tag Mouse Monoclonal Antibody from Signalway Antibody (T519) - Antibodies.comEnlarge Image
Figure 1: Anti-Flag-Tag Antibody (A50042)
1ug Flag fusion protein+ Primary antibody #T519 dilution at 1: 10,000.
Flag-Tag Mouse Monoclonal Antibody from Signalway Antibody (T519) - Antibodies.comEnlarge Image
Figure 2: Anti-Flag-Tag Antibody (A50042)
IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb #T519 at a 1: 2000 dilution (blue DAPI, red anti-Flag).
Flag-Tag Mouse Monoclonal Antibody from Signalway Antibody (T519) - Antibodies.comEnlarge Image
Figure 3: Anti-Flag-Tag Antibody (A50042)
IP antibody use£º5ug Flag Mouse IgG1 per ml Lysate£¬WB 1: 5000 1 ) untransfected 293 cell lysate 2 ) transfected 293 cell lysate with Flag-tag fusion protein 3 ) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4 ) IP (transfected 293+anti- Flag mAb+ Protein G agarose) 5 ) IP (transfected 293+Protein G) 6 ) Recombinant protein (E.coli).
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