Anti-Fibroblast Activation Protein alpha Antibody [DM154] - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM154] antibody to Fibroblast Activation Protein alpha.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM154
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Figure 1: Anti-Fibroblast Activation Protein alpha Antibody [DM154] - BSA and Azide free (A318554)
ELISA plate pre-coated by 1 µg/ml (100 µl/well) Recombinant Human Fibroblast Activation Protein alpha Protein (6×His Tag) (A318116) can bind Anti-Fibroblast Activation Protein alpha Antibody [DM154] - Azide free (A318554) in a linear range of 1-500 µg/ml.
Figure 2: Flow Cytometry - Anti-Fibroblast Activation Protein alpha Antibody [DM154] - BSA and Azide free (A318554)
Flow cytometry analysis with Anti-Fibroblast Activation Protein alpha Antibody [DM154] - Azide free (A318554) on Expi293 cells transfected with human FAP (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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